Transfection Reagents
Miltenyi Biotec, Inc. pMACS LNGFR IRES plasmid vector
Vector for bi-cistronic expression of the truncated human LNGFR surface marker and a protein of interest
Lonza Walkersville Human Stem Cell Nucleofector Kit 1
The Nucleofector 2b Device (or older generations I or II) works with cell type specific kits, each of them dedicated to an individual primary cell. The Human Stem Cell Nucleofector Kit 1 is one of our two kits suited for efficient transfection of human stem cells. Due to significant diversity between different human stem cell clones, we have developed two Nucleofector Kits (1 and 2) and an easy-to-use Nucleofector Starter Kit to determine which of the 2 kits is optimal for a specific clone.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Human Stem Cells0.5 mL Supplement 1, 30 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
HORIZON DISCOVERY BIOSCIENCES LIMITED DHARMAFECT SET OF 4 TRANSFECTI
Dharmafect Set of 4 transfection reagent
Boca Scientific Inc LentiBlast Premium Transduction Enhancer, 1500ul
LentiBlast Premium Transduction Enhancer is designed to increase lentiviral infection and transduction efficiency in a wide range of cell types, from CD34+ hematopoietic stem cells to primary cells or cell lines such as HT1080 or SH SY5Y. LentiBlast Premium is composed of only one reagent, allowing for a new single-step protocol. With LentiBlast Premium, neutralizing electrostatic repulsions between membranes and viral particles and enhancing viral fusion with cell membranes can occur simultaneously. Due to a favorable membrane permeable effect limiting the transmembrane potential changes, LentiBlast Premium is non-toxic and totally compatible with cell viability.
HORIZON DISCOVERY BIOSCIENCES LIMITED SIGLO RED TRANSFECTION INDICAT
NC3826711 SIGLO RED TRANSFECTION INDICAT
Cell Systems Corporation Human Primary Keratinocytes (ACBRI 2K0)
Primary Human Primary Keratinocytes (ACBRI 2K0) were isolated from dispase dissociated dermal tissue. Unlike cells from other providers, Cell Systems primary cells are isolated without positive control antibody labels. Each vial contains ~1 x 10^6 cells with 85-92% viability under ideal conditions. They are available at Passage 3 (<12 cumulative population doublings) and will initiate a Passage 4 cell culture in a 75 cm2 flask. Each vial of cells is shipped with Bac-Off (antibiotic) and CultureBoost (animal derived growth factors) at no additional cost. These cells require specialized low-calcium Keratinocyte media. You can enhance consistency in your research program with reserved lots. Need vials in bulk? Contact Fisher Scientific Sales rep for tailored quotes. Tight Deadline? Cells are available for next day delivery after the purchase order is received. Accelerate Your Research! Try our Attachment Factor (4Z0-201, 4Z0-210) – innovative 5 seconds plating solution for all surfaces.
